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insulin receptor substrate  (Proteintech)


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    Structured Review

    Proteintech insulin receptor substrate
    Insulin Receptor Substrate, supplied by Proteintech, used in various techniques. Bioz Stars score: 98/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/insulin+receptor+substrate/Beclin+1+Fusion+Protein/pm41765454-87-42-41
    Average 98 stars, based on 68 article reviews
    insulin receptor substrate - by Bioz Stars, 2026-10
    98/100 stars

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    Incubation:

    Article Title: Roseburia hominis enriched by baicalin reverses the non-response to metformin via upregulating linolenic acid metabolism
    Article Snippet: .. These membranes were then 533 incubated at 4°C overnight with primary antibodies against AMP-activated protein kinase (AMPK) 534 (Proteintech, Cat# 10929-2-AP, RRID:AB_2169568, 1:4,000), insulin receptor substrate 1 535 (IRS1) (Proteintech, Cat# 17509-1-AP, RRID:AB_10596914, 1:1,000), phospho-AMPKα 536 (Thr172) (40H9) (Cell Signaling Technology, Cat# 2535, RRID:AB_331250, 1:1,000), phospho-537 IRS-1 (Ser636/639) (Cell Signaling Technology, Cat# 2388, RRID:AB_330339, 1:1,000), 538 phosphoenolpyruvate carboxykinase (PCK) (Proteintech, Cat# 16754-1-AP, 539 RRID:AB_2160031, 1:30,000), glucose-6-phosphatase (G6PC) (Proteintech, Cat# 66860-1-Ig, 540 RRID:AB_2882199, 1:5,000), mammalian target of rapamycin (mTOR) (Proteintech, Cat# 541 66888-1-Ig, RRID:AB_2882219, 1:30,000), fatty acid desaturase 2 (FADS2) (Proteintech, Cat# 542 28034-1-AP, RRID:AB_2918142, 1:4,000), phospholipase A2 group (PLA2G) (Proteintech, 543 Cat# 18088-1-AP, RRID:AB_10859777, 1:1,000), acetyl-CoA Carboxylase 1 (ACC1) 544 (Proteintech, Cat# 21923-1-AP, RRID:AB_11042445, 1:4,000), carnitine palmitoyl transferase 545 1 (CPT1) (Proteintech, Cat# 15184-1-AP, RRID:AB_2084676, 1:50,000), glyceraldehyde-3-546 phosphate dehydrogenase (GAPDH) (Proteintech, Cat# 10494-1-AP, RRID:AB_2263076, 547 1:20,000), β-tubulin (Proteintech, Cat# 10094-1-AP, RRID:AB_2210695, 1:10,000). ..

    Article Title: Activation of autophagy through Sirt1/AMPK/mTOR mediates the protective effects of fraxin against insulin resistance and steatosis in rats.
    Article Snippet: Equal amounts of proteins were separated by SDS-PAGE and transferred to 175 PVDF membranes (Millipore, USA). .. Following blocking with 5% milk, the membranes were then 176 incubated overnight at 4°C with primary antibodies against Sirt1 (abcam, USA), AMPKα (CST, 177 USA), p-AMPKα (CST), mTOR (Proteintech), p-mTOR (Ser2448; Proteintech), LC3 178 (Proteintech), ATG5 (Abcam), Beclin 1 (Abcam), p62 (Proteintech), insulin receptor substrate 1 179 (IRS-1; CST), p-IRS-1(Ser612; CST), and p-IRS-1 (Ser307; CST). ..

    Blocking Assay:

    Article Title: Activation of autophagy through Sirt1/AMPK/mTOR mediates the protective effects of fraxin against insulin resistance and steatosis in rats.
    Article Snippet: Equal amounts of proteins were separated by SDS-PAGE and transferred to 175 PVDF membranes (Millipore, USA). .. Following blocking with 5% milk, the membranes were then 176 incubated overnight at 4°C with primary antibodies against Sirt1 (abcam, USA), AMPKα (CST, 177 USA), p-AMPKα (CST), mTOR (Proteintech), p-mTOR (Ser2448; Proteintech), LC3 178 (Proteintech), ATG5 (Abcam), Beclin 1 (Abcam), p62 (Proteintech), insulin receptor substrate 1 179 (IRS-1; CST), p-IRS-1(Ser612; CST), and p-IRS-1 (Ser307; CST). ..



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    VOW, Vaccinium oldhamii water extract; LPS, lipopolysaccharide; p-ERK, <t>phosphorylated</t> extracellular signal-regulated kinase; ERK, extracellular signal-regulated kinase; p-JNK, phosphorylated Jun N-terminal kinase; JNK, Jun N-terminal kinase; p-p38, phosphorylated p38; p-IKKα/β, phosphorylated IKKα/β; NF-κB, nuclear factor kappa-light-chain-enhancer of activated B cells; iNOS, inducible nitric oxide synthase; COX-2, cyclooxygenase-2; NO, nitric oxide.
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    Image Search Results


    VOW, Vaccinium oldhamii water extract; LPS, lipopolysaccharide; p-ERK, phosphorylated extracellular signal-regulated kinase; ERK, extracellular signal-regulated kinase; p-JNK, phosphorylated Jun N-terminal kinase; JNK, Jun N-terminal kinase; p-p38, phosphorylated p38; p-IKKα/β, phosphorylated IKKα/β; NF-κB, nuclear factor kappa-light-chain-enhancer of activated B cells; iNOS, inducible nitric oxide synthase; COX-2, cyclooxygenase-2; NO, nitric oxide.

    Journal: Nutrition Research and Practice

    Article Title: Vaccinium oldhamii fruits improve insulin resistance by inhibiting inflammation in macrophages and adipocytes

    doi: 10.4162/nrp.2025.19.6.839

    Figure Lengend Snippet: VOW, Vaccinium oldhamii water extract; LPS, lipopolysaccharide; p-ERK, phosphorylated extracellular signal-regulated kinase; ERK, extracellular signal-regulated kinase; p-JNK, phosphorylated Jun N-terminal kinase; JNK, Jun N-terminal kinase; p-p38, phosphorylated p38; p-IKKα/β, phosphorylated IKKα/β; NF-κB, nuclear factor kappa-light-chain-enhancer of activated B cells; iNOS, inducible nitric oxide synthase; COX-2, cyclooxygenase-2; NO, nitric oxide.

    Article Snippet: Primary antibodies (phosphorylated extracellular signal-regulated kinase [p-ERK, #4370; Cell Signaling Technology; CST, Beverly, MA, USA], extracellular signal-regulated kinase [ERK, #4695; CST], phosphorylated JNK [p-JNK, #9255; CST], JNK [#9252; CST], phosphorylated p38 [p-p38, #4511; CST], p38 [#8690; CST], phosphorylated IKKα/β [p-IKKα/β, #2697; CST], IKKα [#61294; CST], NF-κB [#8242; CST], inducible nitric oxide synthase [iNOS, #13120; CST], cyclooxygenase-2 [COX-2, #4842; CST], glucose transporter type 4 [GLUT4, #2213; CST], phosphorylated AK strain transforming [p-AKT, #9271; CST], Ak strain transforming [AKT, #9272; CST], phosphorylated phosphatidylinositol 3-kinase [p-PI3K, #4228; CST], phosphatidylinositol 3-kinase [PI3K, #4292; CST], phosphorylated insulin receptor substrate [p-IRS (Ser 307), #2381; CST], p-IRS (Tyr 632) [sc-17196; Santa Cruz, Dallas, TX, USA], and insulin receptor substrate [#2382; CST]) overnight with horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit or goat anti-mouse [1:10,000]).

    Techniques:

    VOW, Vaccinium oldhamii water extract; GLUT4, glucose transporter type 4; p-AKT, phosphorylated AK strain transforming; AKT, AK strain transforming; p-PI3K, phosphorylated phosphatidylinositol 3-kinase; PI3K, phosphatidylinositol 3-kinase; p-IRS, phosphorylated insulin receptor substrate; IRS, insulin receptor substrate.

    Journal: Nutrition Research and Practice

    Article Title: Vaccinium oldhamii fruits improve insulin resistance by inhibiting inflammation in macrophages and adipocytes

    doi: 10.4162/nrp.2025.19.6.839

    Figure Lengend Snippet: VOW, Vaccinium oldhamii water extract; GLUT4, glucose transporter type 4; p-AKT, phosphorylated AK strain transforming; AKT, AK strain transforming; p-PI3K, phosphorylated phosphatidylinositol 3-kinase; PI3K, phosphatidylinositol 3-kinase; p-IRS, phosphorylated insulin receptor substrate; IRS, insulin receptor substrate.

    Article Snippet: Primary antibodies (phosphorylated extracellular signal-regulated kinase [p-ERK, #4370; Cell Signaling Technology; CST, Beverly, MA, USA], extracellular signal-regulated kinase [ERK, #4695; CST], phosphorylated JNK [p-JNK, #9255; CST], JNK [#9252; CST], phosphorylated p38 [p-p38, #4511; CST], p38 [#8690; CST], phosphorylated IKKα/β [p-IKKα/β, #2697; CST], IKKα [#61294; CST], NF-κB [#8242; CST], inducible nitric oxide synthase [iNOS, #13120; CST], cyclooxygenase-2 [COX-2, #4842; CST], glucose transporter type 4 [GLUT4, #2213; CST], phosphorylated AK strain transforming [p-AKT, #9271; CST], Ak strain transforming [AKT, #9272; CST], phosphorylated phosphatidylinositol 3-kinase [p-PI3K, #4228; CST], phosphatidylinositol 3-kinase [PI3K, #4292; CST], phosphorylated insulin receptor substrate [p-IRS (Ser 307), #2381; CST], p-IRS (Tyr 632) [sc-17196; Santa Cruz, Dallas, TX, USA], and insulin receptor substrate [#2382; CST]) overnight with horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit or goat anti-mouse [1:10,000]).

    Techniques:

    MCM, macrophage-conditioned media; VOW, Vaccinium oldhamii water extract; GLUT4, glucose transporter type 4; p-AKT, phosphorylated AK strain transforming; AKT, AK strain transforming; p-PI3K, phosphorylated phosphatidylinositol 3-kinase; PI3K, phosphatidylinositol 3-kinase; p-IRS, phosphorylated insulin receptor substrate; IRS, insulin receptor substrate.

    Journal: Nutrition Research and Practice

    Article Title: Vaccinium oldhamii fruits improve insulin resistance by inhibiting inflammation in macrophages and adipocytes

    doi: 10.4162/nrp.2025.19.6.839

    Figure Lengend Snippet: MCM, macrophage-conditioned media; VOW, Vaccinium oldhamii water extract; GLUT4, glucose transporter type 4; p-AKT, phosphorylated AK strain transforming; AKT, AK strain transforming; p-PI3K, phosphorylated phosphatidylinositol 3-kinase; PI3K, phosphatidylinositol 3-kinase; p-IRS, phosphorylated insulin receptor substrate; IRS, insulin receptor substrate.

    Article Snippet: Primary antibodies (phosphorylated extracellular signal-regulated kinase [p-ERK, #4370; Cell Signaling Technology; CST, Beverly, MA, USA], extracellular signal-regulated kinase [ERK, #4695; CST], phosphorylated JNK [p-JNK, #9255; CST], JNK [#9252; CST], phosphorylated p38 [p-p38, #4511; CST], p38 [#8690; CST], phosphorylated IKKα/β [p-IKKα/β, #2697; CST], IKKα [#61294; CST], NF-κB [#8242; CST], inducible nitric oxide synthase [iNOS, #13120; CST], cyclooxygenase-2 [COX-2, #4842; CST], glucose transporter type 4 [GLUT4, #2213; CST], phosphorylated AK strain transforming [p-AKT, #9271; CST], Ak strain transforming [AKT, #9272; CST], phosphorylated phosphatidylinositol 3-kinase [p-PI3K, #4228; CST], phosphatidylinositol 3-kinase [PI3K, #4292; CST], phosphorylated insulin receptor substrate [p-IRS (Ser 307), #2381; CST], p-IRS (Tyr 632) [sc-17196; Santa Cruz, Dallas, TX, USA], and insulin receptor substrate [#2382; CST]) overnight with horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit or goat anti-mouse [1:10,000]).

    Techniques: